- No interference from gel particles in semen
- No interference from egg yolk or milk in extender
- Easy operation
- 30 sec. analysis time
The NucleoCounter® SP-100™ Sperm Cell Counter offers unique ease of use and effective determination of total sperm concentration and sperm viability in a semen sample from ejaculates or extended doses.
The NucleoCounter® SP-100™ is very simple to operate and the measurements can be performed by operators with only limited training in laboratory work. The compact instrument fits perfectly in any research, quality control or production site.
The measurement range of the NucleoCounter® SP-100™ is virtually unlimited due to dilution prior to the analysis.
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SP-100™ Key features:
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- Easy operation
- 30 sec. analysis time
- No cleaning or calibration
- Excellent reproducibility
- No interference from gel particles in semen
- No interference from egg yolk or milk in extender
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NucleoCounter® SP-100™ – Highest Precision in Counting Sperm Cells
Determination of Cell Viability in Sperm Cell Sample
The NucleoCounter® SP-100™ concept offers determination of cell viability in addition to the total count of cells in the sperm sample. Determination of viability is based on the “PI-exclusion” method (the principle is presented below).
Measuring Sperm Cell Viability
Cell Viability (the membrane integrity) can be measured with the NucleoCounter® SP-100™ in a two-step procedure by measuring the difference between the total cell count and the non-viable cell count (see the figure below).
When a sample is diluted with Reagent S100 a lysis of the viable cell membrane takes place rendering all the cell nuclei susceptible to staining with propidium iodide (PI) resulting in a total cell count.
Non-viable cells diluted with PBS (Phosphate Buffer Saline) are however permeable without treatment and are therefore stained directly with PI, resulting in a non-viable cell count. The difference between the two measurements provides the viable cell count – or % Viability.
| Technical Specifications | |
|---|---|
| Product number | 900-0100 |
| Loading volume | Approximately 60 μl is loaded into the SP1-Cassette™ |
| Measurement volume | 1 μl in the measurement chamber of the SP1-Cassette™ |
| Analysis time | Total count: 30 seconds. Non Viable count: 80 seconds |
| Size | 38 x 26 x 22 cm (W x H x D) |
| Weight | 3 kg / 6.7 lb |
| Software | SemenView™ computer software for documentation and presentation – included |
| Printer | External printer for documentation – optional |
| NucleoCounter® SP-100™ Brochures | ||
|---|---|---|
| No. | Brochure Name | Download |
| 990-0100 | SP-100™ Application Flyer – For total count of sperm cells and viability | Download PDF |
| NucleoCounter® SP-100™ Application Notes | ||
|---|---|---|
| No. | Assay Name | Download |
| 994-0104 | BULLS: Determination of Sperm Density in Ejaculates from Bulls | Download PDF |
| 994-0105 | BULLS: Determination of Sperm Density in Bull Semen Doses for AI | Download PDF |
| 994-0101 | BOARS: Determination of Sperm Density in Ejaculates from Boars | Download PDF |
| 994-0103 | BOARS: Determination of Sperm Cell Density in Boar Semen Doses for AI | Download PDF |
| 994-0106 | STALLIONS: Evaluation of stallion semen | Download PDF |
| NucleoCounter® SP-100™ Documentation | ||
|---|---|---|
| No. | Document Name | Download |
| 991-0100 | NucleoCounter® SP-100™ User’s Guide v1.5 | Download PDF |
| 991-0103 | Software: SemenView™ User’s Guide | Download PDF |
| 994-0030 | LED Life Time Considerations for the NucleoCounters® | Download PDF |
| 991-0104 | NucleoCounter® SP-100™ Addendum to User’s Guide | Download PDF |
| 991-0027 | Installation of SemenView™ on 64-bit OS | Download PDF |
| 994-0102 | NucleoCounter® SP-100™ System. Connecting a PCNEOS Thermal printer | Download PDF |
| / | Installation of NucleoView® 2.x on Windows 7 operating system (32 bit only) | Download PDF |
| / | Certificate of Conformity | Download PDF |
| Consumables | Accessories |
|---|---|
| SP1-Cassette™, 1 box (100 pcs.) | Dispenser 1-10 ml. |
| Reagent S100, 500 ml | Sample Cup 20 ml, 1,000 pcs. |
| Reagent S100, 5l | |
| Isotonic Phosphate-buffered Saline, 5l |
*Note: Purchase the Consumables and Accessories directly in our WebShop.
| NucleoCounter® SP-100™ References |
|---|
| References |
| SP-100™ Reference List |
| Statements |
| Statement from Accelerated Genetics, signed by Jim Martin, Reproductive Specialist |
| JSR Genetics Statement 2013. |
| JSR Genetics Statement 2012. |